Bookedited by Grant J. Jensen.
Contents:
Historical perspective. 3D reconstruction from electron micrographs: a personal account of its development / David DeRosier
Chapter 1. Preparation of 2D crystals of membrane proteins for high-resolution electron crystallography data collection / Priyanka D. Abeyrathne ... [et al.]
Chapter 2. Helical crystallization of soluble and membrane binding proteins / Elizabeth M. Wilson-Kubalek, Joshua S. Chappie, Christopher P. Arthur
Chapter 3. Plunge freezing for electron cryomicroscopy / Megan J. Dobro ... [et al.]
Chapter 4. A Practical guide to the use of monolayer purification and affinity grids / Deborah F. Kelly, Danijela Dukovski, Thomas Walz
Chapter 5. GraFix: stabilization of fragile macromolecular complexes for single particle cryo-EM / Holger Stark
Chapter 6. Cryonegative staining of macromolecular assemblies / Sacha De Carlo, Holger Stark
Chapter 7. Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM / Liguo Wang, Fred J. Sigworth
Chapter 8. Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing / Mark S. Ladinsky
Chapter 9. Site-specific biomolecule labeling with gold clusters / Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
Chapter 10. How to operate a cryo-electron microscope / Jingchuan Sun, Huilin Li
Chapter 11. Collecting electron crystallographic data of two-dimensional protein crystals / Richard K. Hite ... [et al.]
Chapter 12. Automated data collection for electron microscopic tomography / Shawn Q. Zheng, J.W. Sedat, D.A. Agard
Chapter 13. Correlated light and electron cryo-microscopy / Ariane Briegel ... [et al.]
Chapter 14. Phase plates for transmission electron microscopy / Radostin Danev, Kuniaki Nagayama
Chapter 15. Radiation damage in electron cryomicroscopy / Lindsay A. Baker, John L.
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